Multi-well Exosome Libraries for High-throughput In Vitro Cell Activity Assays

Scientists from the Max-Planck-Institute for Multidisciplinary Sciences in Göttingen have developed a method to construct stable EV libraries in standard microtiter plates. These libraries are suitable for high-throughput cell activity assays. By a dry-drop approach purified EVS are immobilized on sterile surfaces while maintaining their full activity. Advantageously, the immobilized EVs can then be frozen and kept at -20 or -80 °C, shipped and thawed again. Since the coated EVS are free of a buffer solution, any kind of cell-based in vitro assay can be performed using the EV library (see figure). The assay is scaled down in volume and requires only limited amounts of testing material. The multi-well format makes it possible to analyze various cellular responses like proliferation, cell viability, differentiation, migration and apoptosis by microscopy or by automated high-throughput assays with multimode plate reader. Preparation of custom-made EV libraries enables scientists to study EV signaling in a parallel, highly reproducible and quantifiable manner.

Ditte et al. (2022) Journal of Extracellular Vesicles. DOI: 10.1002/jev2.12276.

A priority application was filed in 2022.