Bottom-up synthetic virions as a tool to study SARS-CoV-2 infectivity


Prozesse und Methoden (inkl. Screening) : Lebens-Wissenschaften (inkl. Screening)
Prozesse und Methoden (inkl. Screening) : Produktion
Nukleinsäure-, Protein-, Zell-bezogene Technologien : Protein (bezogen)

Ref.-Nr.: 0105-6291-IKF

COVID-19 presents a major threat to human health and its pathogenesis is not yet completely understood. The SARS-CoV-2 virus has been found to be highly mutagenic, which limits well-controlled studies of its infectivity and enforces high biosafety working environments.

Technology

Scientists from the Max-Planck-Institute for Medical Research developed the bottom-up synthesis of synthetic virions consisting of small unilamellar vesicles (SUVs) with a lipid composition that resembles that of the SARS-CoV-2 virus. The lipidic membrane contains NTA-Ni2+ functionalized lipids and can be, therefore, decorated with a Histidine-tagged SARS-CoV-2 Spike protein creating synthetic minimal virions (MiniVs). Consistent with SARS-CoV-2 virions, vesicles present a size and zeta potential comparable to the natural virus. MiniVs are modular, adaptive systems that allow quantitative and flexible assessment of different Spike-variants while controlling the composition and biophysical properties of the virus-mimicking particles. Most important, MiniVs can be precisely programmed and deployed under biosafety level 1 conditions.

We are now looking for a collaboration partner to further develop this exciting project.

 

Patent Information

A European priority establishing patent application was submitted in August 2021.

Publications

  • Staufer, O. et al. (2022). Synthetic virions reveal fatty acid-coupled adaptive immunogenicity of SARS-CoV-2 spike glycoprotein. Nature Communications, accepted for publication
  • Gupta, K. et al. (2022) Structural insights in cell-type specific evolution of intra-host diversity by SARS-CoV-2. Nature Communications, 13(222)
  • Toelzer, C. et a. (2020) Free fatty acid binding pocket in the locked structure of SARS-CoV-2 spike protein. Science, 370(6517)

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Kontaktperson

Patent- & Lizenzmanagerin

Dr. Ingrid Kapser-Fischer

Ernährungswissenschaftlerin, M.Sc.

Telefon: 089 / 29 09 19-19
E-Mail:
kapser-fischer@max-planck-innovation.de