True-to-detail imaging of the spatial arrangement of proteins and other biomolecules in cells and observing molecular processes – GSDIM makes this possible for researchers due to resolutions beyond the diffraction limit. The more insight science gains into these basic processes of life, the better it can find the causes of previously incurable diseases and develop suitable therapies.
One of the strengths of GSDIM is that it uses conventional fluorescence markers to image proteins or other biomolecules within the cells with sharpness down to a few nanometers. This includes fluorophores, which are routinely used in biomedical work, such as fluorescent proteins and rhodamines.
With GSDIM, the fluorescent molecules in the specimen are almost completely switched off using laser light. However, individual molecules spontaneously return to the fluorescent state, while their neighbours remain non-illuminating. In this way, the signals of individual molecules can be acquired sequentially using a highly sensitive camera system and their spatial position in the specimen can be measured and stored. An extremely high-resolution image can then be created from the position of many thousands of molecules. This enables cell components that are situated very close to one another and cannot be resolved using conventional wide field fluorescence microscopy to be spatially separated and sharply reproduced in an image.
"We are glad to continue the already very fruitful cooperation with Prof. Hell and the Max Planck Institute with this groundbreaking and promising technology," explains Dr. Stefan Traeger, head of the Life Science Division at Leica Microsystems. "With GSDIM technology, we have the potential of further expanding our innovation leadership in the market for super-resolution light microscopy and nanoscopy. We want to be able to offer maximum-resolution microscopy in the future to an even wider group of users in the life sciences."
Prof. Stefan Hell explains: "Leica Microsystems was by far the first company to take light microscopy's historical breakthrough of the diffraction limit and implement this in products. We are glad that with GSDIM, Leica is making available another nanoscopic method – which complements STED microscopy - worldwide."
Hell has already invented the 4Pi and STED technologies, which achieve ultra-high resolutions in the nanoscale. For the Leica TCS 4PI Microscope, Leica Microsystems received the German Business Innovation Award in 2005; for STED technology, Prof. Hell was awarded the German Future Prize by Federal President Dr. Horst Köhler in 2006.
About Leica Microsystems
Leica Microsystems is a leading global designer and producer of innovative, high-tech, precision optical systems for the analysis of microstructures. It is one of the market leaders in each of its business areas: Microscopy, Confocal Laser Scanning Microscopy with corresponding Imaging Systems, Specimen Preparation, and Medical Equipment. The company manufactures a broad range of products for numerous applications requiring microscopic imaging, measurement, and analysis. It also offers system solutions for life science including biotechnology and medicine, research and development of raw materials, and industrial quality assurance. The company is represented in over 100 countries with 11 manufacturing facilities in 8 countries, sales and service organizations in 19 countries and an international network of dealers. The international management is headquartered in Wetzlar, Germany.
About Max Planck Innovation
Max Planck Innovation advises and supports scientists of the Max Planck Society in evaluating inventions and filing patent applications. Max Planck Innovation markets patents and technologies to industry and coaches founders of new companies based on research results from Max Planck Institutes. Every year, Max Planck Innovation evaluates about 150 inventions, of which about half lead to the filing of a patent application. Since 2000, Max Planck Innovation advised about 50 spin-offs, closed more than 700 license deals and generated proceeds of more than 140 million Euros for inventors, institutes and the Max Planck Society. As a result, Max Planck Innovation is among the world's most successful technology transfer organizations.
Dr. Bernd Ctortecka
Patent and Licensing Manager
Max Planck Innovation GmbH
Phone: + 49 89 29 09 19-0
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